[Theory of selectivity of RP-LC C18 column and its application].
Yao Xue Xue Bao
; 45(5): 555-9, 2010 May.
Article
em Zh
| MEDLINE
| ID: mdl-20931754
ABSTRACT
In recent years, more and more researchers focus on the selectivity of RP-HPLC columns. Among these columns, C18 column is the most commonly used the column. Yet even the same filler can not give us the same separation effect with the columns of different brands and different types, therefore, it is important to choose the suitable HPLC column for carrying out an experiment. After study in recent years, some column classification, selection methods (e.g. column parameter method, F value method) have been achieved and contributed to the finding of suitable columns and reducing the blindness of column selection. This paper summarizes the advantages of these methods and their limitations in the application process, and gives some advice and expectations to pharmaceutical analysis works based on the works of other researchers.
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Base de dados:
MEDLINE
Assunto principal:
Cromatografia de Fase Reversa
Idioma:
Zh
Revista:
Yao Xue Xue Bao
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
China