Enzyme-linked immunospot assay detection of mumps-specific antibody-secreting B cells as an alternative method of laboratory diagnosis.
Clin Vaccine Immunol
; 18(1): 35-42, 2011 Jan.
Article
em En
| MEDLINE
| ID: mdl-21047998
ABSTRACT
Although high measles, mumps, and rubella (MMR) vaccination coverage has been successful in dramatically reducing mumps disease in the United States, mumps (re)infections occasionally occur in individuals who have been either previously vaccinated or naturally infected. Standard diagnostics that detect virus or virus-specific antibody are dependable for confirming primary mumps infection in immunologically naïve persons, but these methods perform inconsistently for individuals with prior immune exposure. We hypothesized that detection of activated mumps-specific antibody-secreting B cells (ASCs) by enzyme-linked immunospot (ELISPOT) assay could be used as a more reliable diagnostic. To test this, a time course of virus-specific ASC responses was measured by ELISPOT assay following MMR vaccination of 16 previously vaccinated or naturally exposed adult volunteers. Mumps-specific ASCs were detectable in 68% of these individuals at some point during the first 3 weeks following revaccination. In addition, mumps-specific ASCs were detected in 7/7 previously vaccinated individuals who recently had been infected as part of a confirmed mumps outbreak. These data suggest that ELISPOT detection of mumps-specific ASCs has the potential for use as an alternative method of diagnosis when suspect cases cannot be confirmed by detection of IgM or virus. In addition, it was determined that mumps-specific memory B cells are detected at a much lower frequency than measles- or rubella-specific cells, suggesting that mumps infection may not generate robust B-cell memory.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
/
Vacina contra Sarampo-Caxumba-Rubéola
/
ELISPOT
/
Anticorpos Antivirais
/
Caxumba
/
Vírus da Caxumba
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Adult
/
Humans
/
Middle aged
País/Região como assunto:
America do norte
Idioma:
En
Revista:
Clin Vaccine Immunol
Assunto da revista:
ALERGIA E IMUNOLOGIA
/
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos