OXA-23-producing Acinetobacter baumannii: a new hotspot of diversity in Rio de Janeiro?
J Antimicrob Chemother
; 66(1): 62-5, 2011 Jan.
Article
em En
| MEDLINE
| ID: mdl-21051372
ABSTRACT
OBJECTIVES:
this study focused on the population structure of OXA-23-producing Acinetobacter baumannii clinical isolates from Rio de Janeiro, Brazil.METHODS:
the analysis included several genomic typing methods, including PFGE, two multilocus sequence typing (MLST) schemes, sequence group (SG) determination and bla(OXA-51-like) sequencing. The genomic context of the bla(OXA-23) gene was also evaluated using I-CeuI hybridizations and PCR assays.RESULTS:
congruent clustering was obtained revealing four lineages. In accordance, four new sequence types (STs) (ST131, ST132, ST133 and ST134) were obtained with the MLST-OD scheme (associated with the Oxford Database) and four (ST79, ST15 and two new allelic profiles) with the MLST-IP scheme (developed by the Institute Pasteur). Four SGs (SG1, SG4 and two new profiles) were identified, allowing the association of 70% of the isolates with European clone II. bla(OXA-51-like) sequencing revealed the presence of bla(OXA-66), bla(OXA-69), bla(OXA-95) and bla(OXA-132).CONCLUSIONS:
identification of new STs together with new SG profiles are findings suggestive of a local diversity hotspot that is worth exploring.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Variação Genética
/
Beta-Lactamases
/
Infecções por Acinetobacter
/
Acinetobacter baumannii
Tipo de estudo:
Prognostic_studies
Limite:
Humans
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
J Antimicrob Chemother
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Portugal