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[Screening and identification of differential serum proteins related to dermatitis medicamentosa-like of trichloroethylene].
Liu, Jian-jun; Xing, Xiu-mei; Huang, Hai-yan; Yuan, Jian-hui; Xu, Xin-yun; Zhou, Li; Yang, Xi-fei; Fang, Dao-kui.
Afiliação
  • Liu JJ; Toxicology Laboratory, Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China. bio-research@hotmail.com
Zhonghua Yu Fang Yi Xue Za Zhi ; 44(6): 485-9, 2010 Jun.
Article em Zh | MEDLINE | ID: mdl-21055120
OBJECTIVE: To screen and identify differential serum proteins which might be involved in dermatitis medicamentosa-like of trichloroethylene (DMLT). METHODS: Three groups of sera were collected from population exposed to trichloroethylene (TCE) (group I), patients suffering from DMLT (group II), and the healed cases (group III). After removing albumin and IgG in the three pools of sera, a comparative proteomic analysis was carried out. The images were analyzed using ImageMaster Platinum 2D 5.0 to screen the differentially expressed proteins. The protein spots were then subjected to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and tandem mass spectrometry sequencing of tryptic peptides for further identification. RESULTS: The depletion of albumin and IgG greatly increased the number of protein spots to 300 ± 12.Five differential spots were identified, which were complement component C4b, apolipoprotein A-I, apolipoprotein C-III apolipoprotein C-II and transthyretin. Compared with group I, the expression levels of complement component C4b in group III and apolipoprotein C-II in group II were up-regulated (1.352 88-fold, 1.512 14-fold, respectively); compared with group I, the expression levels of apolipoprotein A-I, apolipoprotein C-III and transthyretin in group II were down-regulated (1.601 17-fold, 1.034 49-fold, 1.313 35-fold, respectively). CONCLUSION: The findings of this study show that most of the identified differential proteins are closely related to immunity and liver dysfunction, which provides some evidence on elucidating the mechanisms and screening of biomarkers of TCE intoxication.
Assuntos
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Base de dados: MEDLINE Assunto principal: Tricloroetileno / Proteínas Sanguíneas / Toxidermias / Exposição Ambiental Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Adolescent / Adult / Female / Humans / Male Idioma: Zh Revista: Zhonghua Yu Fang Yi Xue Za Zhi Ano de publicação: 2010 Tipo de documento: Article País de afiliação: China
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Base de dados: MEDLINE Assunto principal: Tricloroetileno / Proteínas Sanguíneas / Toxidermias / Exposição Ambiental Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Adolescent / Adult / Female / Humans / Male Idioma: Zh Revista: Zhonghua Yu Fang Yi Xue Za Zhi Ano de publicação: 2010 Tipo de documento: Article País de afiliação: China