[Study on method of time-resolved fluorescence spectra for rare fluorescence chelate].

In the present paper, the technology of time-resolved fluorescence spectra was investigated in order to improve the sensitivity of the solid-phase time-resolved fluorescence immunoassay. The fluorescence properties, fluorescence intensity and detection method of the novel complexes 4,4'-dibromo-6,6'-bis(N,N-bis(ethoxycarbonylmethyl) amino methyl)-2,2'-bipyridine were investigated through self-developed solid-phase time-resolved fluorescence immunoassay instrument. At the same time, the Eu-TTA-phen and Eu-beta-NTA-TOPO series of Eu(III) compounds were detected.