Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3' â 5' exonuclease activity.
Anal Chem
; 83(2): 463-6, 2011 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-21114271
ABSTRACT
A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3' â 5' exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Fluorescência
/
Exonucleases
/
Quadruplex G
/
Corantes Fluorescentes
/
Violeta Genciana
Limite:
Humans
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2011
Tipo de documento:
Article