Protein tyrosine kinase 7 has a conserved role in Wnt/ß-catenin canonical signalling.
EMBO Rep
; 12(1): 43-9, 2011 Jan.
Article
em En
| MEDLINE
| ID: mdl-21132015
ABSTRACT
The receptor protein tyrosine kinase 7 (PTK7) was recently shown to participate in noncanonical Wnt/planar cell polarity signalling during mouse and frog embryonic development. In this study, we report that PTK7 interacts with ß-catenin in a yeast two-hybrid assay and mammalian cells. PTK7-deficient cells exhibit weakened ß-catenin/T-cell factor transcriptional activity on Wnt3a stimulation. Furthermore, Xenopus PTK7 is required for the formation of Spemann's organizer and for Siamois promoter activation, events that require ß-catenin transcriptional activity. Using epistatic assays, we demonstrate that PTK7 functions upstream from glycogen synthase kinase 3. Taken together, our data reveal a new and conserved role for PTK7 in the Wnt canonical signalling pathway.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Receptores Proteína Tirosina Quinases
/
Proteínas Wnt
/
Beta Catenina
Limite:
Animals
/
Humans
Idioma:
En
Revista:
EMBO Rep
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2011
Tipo de documento:
Article