Euonymus alatus extract attenuates LPS-induced NF-κB activation via IKKß inhibition in RAW 264.7 cells.
J Ethnopharmacol
; 134(2): 288-93, 2011 Mar 24.
Article
em En
| MEDLINE
| ID: mdl-21182917
ABSTRACT
AIM OF THE STUDY The present study was performed to investigate the underlying mechanisms of anti-inflammatory effects with the extract of Euonymus alatus (EEA), and specially focused on nuclear factor κB (NF-κB) signaling pathway by targeting the IκB kinase ß (IKKß). MATERIALS AND METHODS:
The effect of EEA for IKKß activity was analyzed using an immobilized metal affinity for phosphochemicals (IMAP)-based time-resolved fluorescence resonance energy transfer (TR-FRET) assay. The effect of EEA on lipopolysaccharide (LPS)-induced NF-κB activation in murine macrophage RAW 264.7 cells with western blotting and immunofluorescent staining was evaluated.RESULTS:
IKKß studies based on IMAP-TR-FRET showed that EEA possesses a potent IKKß inhibitory activity with IC(50) value of 11.83µg/ml. EEA (10, 30µg/ml) also attenuated the LPS-induced IκBα phosphorylation/degradation, NF-κB translocation and subsequent NO synthesis in RAW 264.7 cells.CONCLUSIONS:
These results suggest that EEA abrogates LPS-induced NF-κB signaling pathway by targeting the IKKß in RAW 264.7 cells and these properties may provide a molecular basis for understanding the inhibitory effects of EEA on LPS-mediated inflammation.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
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NF-kappa B
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Euonymus
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Quinase I-kappa B
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Macrófagos
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Anti-Inflamatórios
Limite:
Animals
Idioma:
En
Revista:
J Ethnopharmacol
Ano de publicação:
2011
Tipo de documento:
Article