Metabolic production of a novel polymer feedstock, 3-carboxy muconate, from vanillin.
Appl Microbiol Biotechnol
; 90(1): 107-16, 2011 Apr.
Article
em En
| MEDLINE
| ID: mdl-21221570
ABSTRACT
Vanillin can be produced on a commercial scale by depolymerising renewable lignin. One product of microbial metabolism of vanillin by common soil microbes, such as Acinetobacter baylyi, is a tricarboxylic acid with a butadiene backbone known as 3-carboxy muconate (3CM). Three enzymes, 4-hydroxy benzaldehyde dehydrogenase, vanillate monooxygenase and protocatechuate 3,4-dioxygenase, catalyse the biotransformation of vanillin to 3CM. These three enzymes were metabolically engineered into an Escherichia coli host, giving a biocatalyst that converted vanillin into 3CM. The biocatalyst was found to give 100% yield of 3CM from 1 mM of vanillin after 39 h. The rate-limiting reaction was identified as the conversion of vanillate to 3,4-dihydroxybenzoate catalysed by vanillate monooxygenase. Low expression of the reductase subunit of this enzyme was identified as contributing to the reduced rate of this reaction. Proof of principle of a novel application for 3CM was demonstrated when it was converted into a trimethyl ester derivative and copolymerised with styrene.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ácido Sórbico
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Benzaldeídos
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Biopolímeros
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Escherichia coli
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Austrália