Regulation of urea synthesis in sublobular regions of the liver lobule by oxygen.
Biochim Biophys Acta
; 1036(3): 242-4, 1990 Dec 06.
Article
em En
| MEDLINE
| ID: mdl-2124141
ABSTRACT
Periportal and pericentral regions of the liver lobule were isolated from perfused rat liver using a micropunch and incubated in Krebs-Henseleit buffer (pH 7.6) containing 2% poly(ethylene glycol) in Eagle's basal medium, PMSF (50 micrograms/ml) and leupeptin (20 micrograms/ml) for 2 h at 25 degrees C under and O2/CO2 (955%) gas phase. Maximal rates of urea production from ammonium chloride were 96.4 +/- 8.7 and 32.8 +/- 5.4 mumol/g per h at 800 and 200 microM O2. Thus, urea synthesis was 2-3-times greater at high than low O2 tension in plugs from periportal and pericentral regions of the liver lobule.
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Base de dados:
MEDLINE
Assunto principal:
Oxigênio
/
Ureia
/
Fígado
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1990
Tipo de documento:
Article