Comparison of three different immunoassay methods for the evaluation of intact parathyroid hormone levels in hemodialysis patients.
Scand J Clin Lab Invest
; 71(3): 227-31, 2011 May.
Article
em En
| MEDLINE
| ID: mdl-21294669
ABSTRACT
BACKGROUND:
Intact parathyroid hormone (iPTH) assays react with the non-(1-84) molecular form of PTH. This form behaves as a carboxy-terminal fragment and accumulates during renal failure. We wanted to examine the variation of iPTH levels between the more commonly used different immunoassay methods in hemodialysis patients.METHODS:
Our study was designed to compare three commercial second-generation immunoassays based on electrochemiluminescent immunoassay (ECLIA), enzyme immunoassay (EIA) and immunoradiometric assay (IRMA) for intact PTH. The serum samples from 88 patients were collected and the iPTH concentrations measured.RESULTS:
The median iPTH (IRMA) concentration (99 pg/mL) was lower than both median iPTH (ECLIA) concentration (290.5 pg/ml; p < 0.001) and iPTH (EIA) concentration (369 pg/mL; p < 0.001). The Bland-Altman graphs, which are plots of the percentage differences between the two methods against their mean, suggested that the IRMA methods are not in agreement with the other methods.CONCLUSION:
It would be useful to reduce the variability among the methods with the use of a more standardized calibrator and of the same specific antibodies that only recognize the active PTH molecule.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Hormônio Paratireóideo
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Diálise Renal
Tipo de estudo:
Evaluation_studies
Limite:
Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Scand J Clin Lab Invest
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Turquia