Bupivacaine induces reactive oxygen species production via activation of the AMP-activated protein kinase-dependent pathway.
Pharmacology
; 87(3-4): 121-9, 2011.
Article
em En
| MEDLINE
| ID: mdl-21304223
ABSTRACT
AIMS:
It was our aim to investigate whether AMP-activated protein kinase (AMPK) mediates the considerable increase in reactive oxygen species (ROS) and cell apoptosis induced by bupivacaine in the human neuroblastoma cell line SH-SY5Y.METHODS:
The recombinant plasmids pGPU6/GFP/Neo-shRNA AMPKα2 and pEGFP-N1-AMPKα2 were constructed and transfected into the SH-SY5Y cell line. The expression of AMPKα2 was determined by RT-PCR and Western blot after transfection. The SH-SY5Y cells transfected with recombinant plasmid were exposed to 1 mmol/l bupivacaine. Cell viability, intracellular ROS and apoptosis were determined.RESULTS:
The plasmid pEGFP-N1-AMPKα2 can upregulate the expression of AMPKα2, and the pGPU6/GFP/Neo-shRNA AMPKα2 can downregulate the expression of AMPKα2 in cells. Inhibition of AMPKα2 expression attenuated ROS production and cell apoptosis, and overexpression of AMPKα2 promoted ROS production and cell apoptosis after bupivacaine treatment.CONCLUSION:
AMPK probably mediated ROS production and cell apoptosis induced by bupivacaine.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bupivacaína
/
Espécies Reativas de Oxigênio
/
Proteínas Quinases Ativadas por AMP
/
Anestésicos Locais
Limite:
Humans
Idioma:
En
Revista:
Pharmacology
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
China