Semiautomated preparation of DNA templates for large-scale sequencing projects.
DNA Seq
; 1(1): 73-8, 1990.
Article
em En
| MEDLINE
| ID: mdl-2132961
ABSTRACT
The rate limiting step in a large-scale sequencing project is the generation of single-stranded DNA templates. We describe a fast, semiautomated procedure, using 96-well microtitre plates, in which 192 templates can be readily prepared in 1 day. The technique can be carried out manually or can be semiautomated using a robot pipetting device. We also provide evidence for the reliability and applicability of this method to a large-scale sequencing project.
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Base de dados:
MEDLINE
Assunto principal:
Moldes Genéticos
/
DNA Viral
/
Sequência de Bases
Idioma:
En
Revista:
DNA Seq
Assunto da revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Ano de publicação:
1990
Tipo de documento:
Article