Indirubin-3'-monoxime, a derivative of a chinese antileukemia medicine, inhibits angiogenesis.
J Cell Biochem
; 112(5): 1384-91, 2011 May.
Article
em En
| MEDLINE
| ID: mdl-21337385
ABSTRACT
Although the antiangiogenic activity of indirubin-3-monoxime (I3M), a derivative of a Chinese anti-leukemia medicine, has been demonstrated using transgenic zebrafish, the detail molecular mechanism has not been elicited. To further establish its role in antiangiogenic activity, we tested its potential against human umbilical vein endothelial cells (HUVECs) and the in vivo Matrigel plug model was applied to evaluate new vessel formation. We also investigated the molecular mechanisms of I3M-induced antiangiogenic effects in HUVECs. We found that I3M significantly inhibited HUVEC proliferation (2.5-20 µM), migration (2.5-20 µM), and tube formation (10-20 µM) in HUVECs. The number of microvessels growing from the aortic rings was suppressed by I3M treatment. Moreover, I3M suppressed neovascularization in Matrigel plugs in mice. The underlying antiangiogenic mechanism of I3M was correlated with down-regulation of the vascular endothelial growth factor receptor-2 activation, at least a part. These findings emphasize the potential use of I3M in pathological situations involving stimulated angiogenesis, such as tumor development.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oximas
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Medicamentos de Ervas Chinesas
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Inibidores da Angiogênese
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Receptor 2 de Fatores de Crescimento do Endotélio Vascular
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Indóis
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
Idioma:
En
Revista:
J Cell Biochem
Ano de publicação:
2011
Tipo de documento:
Article