Effects of single and multi walled carbon nanotubes on macrophages: cyto and genotoxicity and electron microscopy.
Mutat Res
; 722(1): 20-31, 2011 May 18.
Article
em En
| MEDLINE
| ID: mdl-21382506
ABSTRACT
Production of nanotechnology-based materials is increasing worldwide it is essential to evaluate their potential toxicity. Among these nanomaterials, carbon nanotubes (CNTs) have tremendous potential in many areas of research and applications. We have investigated the cyto- and genotoxic effects of single and multi-walled CNTs (SWCNTs, MWCNTs) and carbon black (CB) on the mouse macrophage cell line RAW 264.7. Specifically we have investigated inflammatory response, release of tumor necrosis factor-α (TNF-α), intracellular reactive oxygen species (ROS) production, cell death (both necrosis and apoptosis), chromosomal aberrations and cellular ultrastructural alteration caused by CB, MWCNTs and SWCNTs. Our data confirm that both CNTs and CB are cyto and geno-toxic to RAW 264.7 mouse macrophages. CNTs exposure induced ROS release, necrosis and chromosomal aberrations but did not cause an inflammatory response. In addition CNTs induce ultrastructural damage and apoptosis. CNTs penetrate the cell membrane and individual MWCNTs are seen associated with the nuclear envelope.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nanotubos de Carbono
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Macrófagos
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Mutagênicos
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Mutat Res
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Itália