Cell-mediated transfer of catalase nanoparticles from macrophages to brain endothelial, glial and neuronal cells.
Nanomedicine (Lond)
; 6(7): 1215-30, 2011 Sep.
Article
em En
| MEDLINE
| ID: mdl-21449849
ABSTRACT
BACKGROUND:
Our laboratories forged the concept of macrophage delivery of protein antioxidants to attenuate neuroinflammation and nigrostriatal neurodegeneration in Parkinson's disease. Notably, the delivery of the redox enzyme, catalase, incorporated into a polyion complex micelle ('nanozyme') by bone marrow-derived macrophages protected nigrostriatum against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine intoxication. Nonetheless, how macrophage delivery of nanozyme increases the efficacy of catalase remains unknown.METHODS:
In this study, we examined the transfer of nanozyme from macrophages to brain microvessel endothelial cells, neurons and astrocytes.RESULTS:
Facilitated transport of the nanozyme from macrophages to endothelial, neuronal and glial target cells occurred through endocytosis-independent mechanisms that involved fusion of cellular membranes, macrophage bridging conduits and nanozyme lipid coatings. Nanozyme transfer was operative across an artificial blood-brain barrier and showed efficient reactive oxygen species decomposition.CONCLUSION:
This is the first demonstration, to our knowledge, that drug-loaded macrophages discharge particles to contiguous target cells for therapeutic brain enzyme delivery. The data shown are of potential value for the treatment of neurodegenerative disorders and notably, Parkinson's disease.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
/
Catalase
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Nanopartículas
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Macrófagos
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Antioxidantes
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Nanomedicine (Lond)
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos