Cytogenetic investigations on a cell line derived from a carcinoma arising in a salivary gland pleomorphic adenoma.
Cancer Genet Cytogenet
; 44(2): 253-62, 1990 Feb.
Article
em En
| MEDLINE
| ID: mdl-2153441
ABSTRACT
This article discusses the results of cytogenetic investigations of a cell line derived from a malignant tumor arising in a benign salivary gland pleomorphic adenoma. Initial karyotypic studies became possible with cells of the second in vitro passage and revealed the existence of hypertetraploidy. Furthermore, a marked polysomy 7 and a translocation involving 12q13-15, a breakpoint also frequently seen in benign pleomorphic adenomas, were noteworthy. During long term culture, the number of chromosomes per cell decreased as well as the number of copies of chromosome 7. Cell tumorigenicity was proved by heterotransplantation to nude mice. The resulting tumors were karyotyped again. No significant changes of the chromosome number or of the degree of polysomy 7 were found compared to the cells before heterotransplantation. In contrast, the cells from the nude mice tumors showed a remarkable number of different isochromosomes probably indicating an unknown factor supporting the generation of isochromosomes. The consistent presence of the t(12;?)(q13-15;?) makes the cell line well suited for molecular studies of this breakpoint region.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias das Glândulas Salivares
/
Carcinoma
/
Aberrações Cromossômicas
/
Adenoma Pleomorfo
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Cancer Genet Cytogenet
Ano de publicação:
1990
Tipo de documento:
Article