Probing conformational states of modified helix 69 in 50S ribosomes.
J Am Chem Soc
; 133(22): 8396-9, 2011 Jun 08.
Article
em En
| MEDLINE
| ID: mdl-21557607
ABSTRACT
The movement of the small ribosomal subunit (30S) relative to the large ribosomal subunit (50S) during translation is widely known, but many molecular details and roles of rRNA and proteins in this process are still undefined, especially in solution models. The functional relationship of modified nucleotides to ribosome activity is one such enigma. To better understand ribosome dynamics and the influence of modified nucleotides on such processes, the focus of this work was helix 69 of 23S rRNA, which contains three pseudouridine residues in its loop region. Ribosome probing experiments with dimethylsulfate revealed that specific base accessibilities and individual nucleotide conformations in helix 69 are influenced differently by pH, temperature, magnesium, and the presence of pseudouridine modifications.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Ribossômico 23S
/
Escherichia coli
Idioma:
En
Revista:
J Am Chem Soc
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos