Methods for engineering sulfate reducing bacteria of the genus Desulfovibrio.
Methods Enzymol
; 497: 503-17, 2011.
Article
em En
| MEDLINE
| ID: mdl-21601101
ABSTRACT
Sulfate reducing bacteria (SRB) are physiologically important given their nearly ubiquitous presence and have important applications in the areas of bioremediation and bioenergy. This chapter provides details on the steps used for homologous-recombination mediated chromosomal manipulation of Desulfovibrio vulgaris Hildenborough, a well-studied sulfate reducer. More specifically, we focus on the implementation of a "parts" based approach for suicide vector assembly, important aspects of anaerobic culturing, choices for antibiotic selection, electroporation-based DNA transformation, as well as tools for screening and verifying genetically modified constructs. These methods, which in principle may be extended to other SRB, are applicable for functional genomics investigations, as well as metabolic engineering manipulations.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sulfatos
/
Engenharia Genética
/
Cromossomos Bacterianos
/
Desulfovibrio
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos