Comparison of exendin-4 on beta-cell replication in mouse and human islet grafts.
Transpl Int
; 24(8): 856-64, 2011 Aug.
Article
em En
| MEDLINE
| ID: mdl-21627696
ABSTRACT
Exendin-4 can stimulate ß-cell replication in mice. Whether it can stimulate ß-cell replication in human islet grafts remains unknown. Therefore, we compared the effects of exendin-4 on ß-cell replication in mouse and human islet grafts. Islets, isolated from mouse and human donors at different ages, were transplanted into diabetic mice and/or diabetic nude mice that were given bromodeoxyuridine (BrdU) with or without exendin-4. At 4 weeks post-transplantation, islet grafts were removed for insulin and BrdU staining and quantification of insulin(+)/BrdU(+) cells. Although diabetes was reversed in all mice transplanting syngeneic mouse islets from young or old donors, normoglycemia was achieved significantly faster in exendin-4 treated mice. Mouse islet grafts in exendin-4 treated mice had significantly more insulin(+)/BrdU(+) ß cells than in untreated mice (P < 0.01). Human islet grafts from ≤22-year-old donors had more insulin(+)/BrdU(+) ß cells in exendin-4 treated mice than that in untreated mice (P < 0.01). However, human islet grafts from ≥35-year-old donors contained few insulin(+)/BrdU(+) ß cells in exendin-4 treated or untreated mice. Our data demonstrated that the capacity for ß-cell replication in mouse and human islet grafts is different with and without exendin-4 treatment and indicated that GLP-1 agonists can stimulate ß-cell replication in human islets from young donors.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
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Peçonhas
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Transplante das Ilhotas Pancreáticas
/
Células Secretoras de Insulina
Limite:
Adolescent
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Adult
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Animals
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Transpl Int
Assunto da revista:
TRANSPLANTE
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos