Oxidation and loss of heme in soluble guanylyl cyclase from Manduca sexta.
Biochemistry
; 50(26): 5813-5, 2011 Jul 05.
Article
em En
| MEDLINE
| ID: mdl-21639146
ABSTRACT
Oxidation and loss of heme in soluble guanylyl/guanylate cyclase (sGC), the nitric oxide receptor, is thought to be a major contributor to cardiovascular disease and is the target of compounds BAY 58-2667 and HMR1766. Using spectroelectrochemical titration, we found a truncated sGC to be highly stable in the ferrous state (234 mV) and to bind ferrous heme tightly even in the presence of NO, despite the NO-induced release of the proximal histidine. In contrast, oxidized sGC readily loses ferric heme to myoglobin (0.47 ± 0.02 h(-1)). Peroxynitrite, the presumed cellular oxidant, readily oxidizes sGC in 5 mM glutathione.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Receptores Citoplasmáticos e Nucleares
/
Manduca
/
Guanilato Ciclase
/
Heme
Limite:
Animals
Idioma:
En
Revista:
Biochemistry
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos