Combinatorial discovery of fluorescent pharmacophores by multicomponent reactions in droplet arrays.
J Am Chem Soc
; 133(26): 10058-61, 2011 Jul 06.
Article
em En
| MEDLINE
| ID: mdl-21644551
ABSTRACT
Fluorescence imaging in clinical diagnostics and biomedical research relies to a great extent on the use of small organic fluorescent probes. Because of the difficulty of combining fluorescent and molecular-recognition properties, the development of such probes has been severely restricted to a number of well-known fluorescent scaffolds. Here we demonstrate that autofluorescing druglike molecules are a valuable source of bioimaging probes. Combinatorial synthesis and screening of chemical libraries in droplet microarrays allowed the identification of new types of fluorophores. Their concise and clean assembly by a multicomponent reaction presents a unique potential for the one-step synthesis of thousands of structurally diverse fluorescent molecules. Because they are based upon a druglike scaffold, these fluorophores retain their molecular recognition potential and can be used to design specific imaging probes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Química Combinatória
/
Análise em Microsséries
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Descoberta de Drogas
/
Corantes Fluorescentes
Idioma:
En
Revista:
J Am Chem Soc
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
França