Down-regulation of ß3-integrin inhibits bone metastasis of small cell lung cancer.
Mol Biol Rep
; 39(3): 3029-35, 2012 Mar.
Article
em En
| MEDLINE
| ID: mdl-21678053
ABSTRACT
Bone is one of the most frequent targets of small cell lung cancer (SCLC) metastasis, but the molecular mechanism remains unclear. ß3-integrin plays an important role in invasion of various kinds of tumors. Yet, its role in bone-metastasis of SCLC is still unknown. In this study, we first examined the expression of ß3-integrin in SBC-5 and SBC-3 cells by real-time PCR, western blot and immunofluorescence. We found that, compared to none bone-metastatic SBC-3 cells, ß3-integrin was highly expressed in SBC-5 cells, a specific bone-metastatic SCLC cells line characterized in our previous study. We next constructed ß3-integrin siRNA and transfected SBC-5 cell line, and found that ß3-integrin siRNA significantly down-regulated the ß3-integrin mRNA level and protein expression in SBC-5 cell line. We further found that inhibition of ß3-integrin significantly reduced tumor cell proliferation and induced apoptosis. In addition, the ß3-integrin down-regulated cells presented significant decrease in cell adhesion, migration and invasion activity. Our results suggest the ß3-integrin has an essential effect on tumor cell proliferation and progression, and may be a potential therapeutic target for the prevention of skeletal metastases of lung cancer.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Ósseas
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Regulação Neoplásica da Expressão Gênica
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Integrina beta3
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Carcinoma de Pequenas Células do Pulmão
Limite:
Humans
Idioma:
En
Revista:
Mol Biol Rep
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China