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Down-regulation of ß3-integrin inhibits bone metastasis of small cell lung cancer.
Li, Na; Zhang, Jian-ping; Guo, Shan; Min, Jie; Liu, Li-li; Su, Hai-chuan; Feng, Ying-ming; Zhang, He-long.
Afiliação
  • Li N; Department of Oncology, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China.
Mol Biol Rep ; 39(3): 3029-35, 2012 Mar.
Article em En | MEDLINE | ID: mdl-21678053
ABSTRACT
Bone is one of the most frequent targets of small cell lung cancer (SCLC) metastasis, but the molecular mechanism remains unclear. ß3-integrin plays an important role in invasion of various kinds of tumors. Yet, its role in bone-metastasis of SCLC is still unknown. In this study, we first examined the expression of ß3-integrin in SBC-5 and SBC-3 cells by real-time PCR, western blot and immunofluorescence. We found that, compared to none bone-metastatic SBC-3 cells, ß3-integrin was highly expressed in SBC-5 cells, a specific bone-metastatic SCLC cells line characterized in our previous study. We next constructed ß3-integrin siRNA and transfected SBC-5 cell line, and found that ß3-integrin siRNA significantly down-regulated the ß3-integrin mRNA level and protein expression in SBC-5 cell line. We further found that inhibition of ß3-integrin significantly reduced tumor cell proliferation and induced apoptosis. In addition, the ß3-integrin down-regulated cells presented significant decrease in cell adhesion, migration and invasion activity. Our results suggest the ß3-integrin has an essential effect on tumor cell proliferation and progression, and may be a potential therapeutic target for the prevention of skeletal metastases of lung cancer.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Ósseas / Regulação Neoplásica da Expressão Gênica / Integrina beta3 / Carcinoma de Pequenas Células do Pulmão Limite: Humans Idioma: En Revista: Mol Biol Rep Ano de publicação: 2012 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Ósseas / Regulação Neoplásica da Expressão Gênica / Integrina beta3 / Carcinoma de Pequenas Células do Pulmão Limite: Humans Idioma: En Revista: Mol Biol Rep Ano de publicação: 2012 Tipo de documento: Article País de afiliação: China