A targeted proteomics-based pipeline for verification of biomarkers in plasma.
Nat Biotechnol
; 29(7): 625-34, 2011 Jun 19.
Article
em En
| MEDLINE
| ID: mdl-21685906
ABSTRACT
High-throughput technologies can now identify hundreds of candidate protein biomarkers for any disease with relative ease. However, because there are no assays for the majority of proteins and de novo immunoassay development is prohibitively expensive, few candidate biomarkers are tested in clinical studies. We tested whether the analytical performance of a biomarker identification pipeline based on targeted mass spectrometry would be sufficient for data-dependent prioritization of candidate biomarkers, de novo development of assays and multiplexed biomarker verification. We used a data-dependent triage process to prioritize a subset of putative plasma biomarkers from >1,000 candidates previously identified using a mouse model of breast cancer. Eighty-eight novel quantitative assays based on selected reaction monitoring mass spectrometry were developed, multiplexed and evaluated in 80 plasma samples. Thirty-six proteins were verified as being elevated in the plasma of tumor-bearing animals. The analytical performance of this pipeline suggests that it should support the use of an analogous approach with human samples.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
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Análise Química do Sangue
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Mapeamento de Peptídeos
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Biomarcadores Tumorais
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Proteoma
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Proteínas de Neoplasias
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Neoplasias Experimentais
Limite:
Animals
Idioma:
En
Revista:
Nat Biotechnol
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos