Characterization of a novel proteinous toxin from sea anemone Actineria villosa.
Protein J
; 30(6): 422-8, 2011 Aug.
Article
em En
| MEDLINE
| ID: mdl-21769623
ABSTRACT
The sea anemone Actineria villosa expresses a lethal protein toxin. We isolated a novel 120-kDa protein, Avt120, from partially purified toxin and found it to possess extremely strong lethal activity. The 3,453-bp Avt120 gene translates to a 995-amino acid protein. The 50% lethal dose (LD(50)) of purified Avt120 in mice was 85.17 ng. Among several tested cell lines, Colo205 cells were most sensitive to Avt120 50% of them were damaged by 38.4 ng/mL Avt120. Avt120 exerted ATP degradation activity (10 µmol ATP h(-1) mg(-1)), which was strongly inhibited by ganglioside GM1 to decrease the cytotoxicity of Avt120.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Anêmonas-do-Mar
/
Venenos de Cnidários
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Protein J
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Japão