Gene expression and inducibility of the aryl hydrocarbon receptor-dependent pathway in cultured bovine blood lymphocytes.
Toxicol Lett
; 206(2): 204-9, 2011 Oct 10.
Article
em En
| MEDLINE
| ID: mdl-21803134
ABSTRACT
The exposure to dioxin-like (DL) compounds, an important class of persistent environmental pollutants, results in the altered expression of target genes. This occurs through the binding to the aryl hydrocarbon receptor (AhR), the subsequent dimerization with the AhR nuclear translocator (ARNT), and the binding of the complex to DNA responsive elements. A number of genes are up-regulated, including, among others, the AhR repressor (AHRR) and several biotransformation enzymes, such as the members of CYP1 family and NAD(P)H-quinone oxidoreductase (NOQ1). The expression and the inducibility of the above genes were investigated in mitogen-stimulated cultured blood lymphocytes from cattle, which represent a notable source of DL-compound human exposure through dairy products and meat. As assessed by real-time PCR, all the examined genes except CYP1A2 and NQO1 were detected under basal conditions. Cell exposure to the DL-compounds PCB126 or PCB77 in the 10(-6)-10(-9)M concentration range resulted in a 2-4-fold induction of CYPIA1 and CYP1B1, which was antagonized by α-naphthoflavone or PCB153. This study demonstrates for the first time the presence and inducibility of the AhR pathway in easily accessible cells like bovine peripheral lymphocytes and prompts further investigations to verify whether similar changes could occur under in vivo conditions.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Linfócitos
/
Transdução de Sinais
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Regulação da Expressão Gênica
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Bifenilos Policlorados
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Receptores de Hidrocarboneto Arílico
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Poluentes Ambientais
Limite:
Animals
Idioma:
En
Revista:
Toxicol Lett
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Itália