Lactoferrin-derived antimicrobial peptide induces a micellar cubic phase in a model membrane system.
Biophys J
; 101(3): L20-2, 2011 Aug 03.
Article
em En
| MEDLINE
| ID: mdl-21806917
ABSTRACT
The observation of a micellar cubic phase is reported for a mixture of an antimicrobial peptide from the Lactoferrin family, LFampin 265-284, and a model membrane system of dimyristoylphosphatidylcholine/dimyristoylphosphatidylglycerol (31), as derived from small-angle x-ray diffraction (SAXD) measurements. The system shows remarkable thermotropic polymorphism the peptide disrupts the lipid bilayer, forming a cubic phase of the space group Pm3n (t < 28°C), and as the temperature increases it shows a complex phase behavior (not fully clarified by SAXD). The onset, volume fraction of each phase, and phase parameters are seen to vary with peptide/lipid ratio and temperature. The obtained SAXD data represent the first experimental evidence, to our knowledge, of a micellar cubic phase in the context of antimicrobial peptide/membrane interaction. We propose that the micellization of the membrane according to the carpet model, for long proposed as a possible mechanism of action, can go through the formation of a cubic micellar phase.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Membrana Celular
/
Peptídeos Catiônicos Antimicrobianos
/
Lactoferrina
/
Micelas
Idioma:
En
Revista:
Biophys J
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Portugal