Efficient in vitro myogenic reprogramming of human primary mesenchymal stem cells and endothelial cells by Myf5.
Biol Cell
; 103(11): 531-42, 2011 Nov.
Article
em En
| MEDLINE
| ID: mdl-21810080
ABSTRACT
BACKGROUND INFORMATION The identification of a source of stem cells able to regenerate skeletal muscle was the goal of numerous studies with the aim to develop new therapeutic approaches for genetic muscle diseases or muscle injuries. A series of studies have demonstrated that stem cells derived from various tissues may have a role in the regeneration of damaged muscles, but this contribution is always very weak. Thus we established a project aiming to reprogramme non-muscle cells into the skeletal striated differentiation pathway. RESULTS:
We transduced several human primary adult stem or progenitor cells using a recombinant lentivirus containing the coding sequence of the Myf5 gene considered as a master gene for the determination of skeletal striated muscle. These original results are the first demonstration of a myogenic conversion of human mesenchymal and endothelial cells by Myf5.CONCLUSIONS:
The procedure described in the present paper could be used to develop new research protocols with the prospect of using these cells as therapeutic agents.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Mioblastos
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Células Endoteliais
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Fator Regulador Miogênico 5
/
Reprogramação Celular
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Células-Tronco Mesenquimais
Tipo de estudo:
Guideline
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Biol Cell
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
França