[Effect of ginsenoside Rg1 on the spatial learning-memory ability in dementia rats after transplanted with bone marrow mesenchymal stem cells].
Zhongguo Zhong Xi Yi Jie He Za Zhi
; 31(6): 799-802, 2011 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-21823427
ABSTRACT
OBJECTIVE:
To study the effect and mechanism of ginsenoside Rg1 on the spatial learning-memory ability in rats with Alzheimer's disease after transplanted with bone marrow mesenchymal stem cells (BMSCs).METHODS:
Using digital randomization table method, seventy-five male SD rats were divided into the bilateral FF transection model group (as the model group ambi-hippocampal fimbria-fomix transected), the sham-operative control group (the SOC group receiving the same modeling process as the model group, but without ambi-hippocampal fimbria-fomix transected), the ginsenoside Rg1 treatment group (as the treatment group Two weeks after modeling ginsenoside Rg1 was peritoneally injected at the dose of 5 mg/kg, once daily for four weeks in total), the BMSCs transplanted treatment group [as the control group Two weeks after modeling every rat received transplantation of BMSCs (10 microL, 1 x 10(6) cells)], and the ginsenoside Rg1 + BMSCs treatment group (as the combination group They received both transplantation of BMSCs and peritoneal injection of ginsenoside Rgl). The spatial learning-memory ability of rats was detected by Morris water maze and the escape latency (s) was recorded, mRNA expression of nerve growth factor (NGF) was detected using Real-time PCR.RESULTS:
Six weeks after the hippocampal fimbria-fomix (FF) transection, the escape latency o feach medication group was obviously shorter than that of the model group, and the spatial learning-memory ability of dementia rats was somewhat improved. The spatial learning-memory ability of rats in the combination group was (29.95 +/- 2.03) and the mRNA expression level of NGF was (1.13 +/- 0.15), better than those in the BMSCs group (44.36 +/- 1.43, 0.78 +/- 0.09, P<0.05).CONCLUSIONS:
Ginsenoside Rg1 could strengthen the spatial learning-memory ability in dementia rats after transplanted with BMSCs. Its mechanism might be possibly correlated with up-regulating mRNA expression of NGF in basal forebrain after BMSCs transplantation.
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Base de dados:
MEDLINE
Assunto principal:
Demência
/
Ginsenosídeos
/
Aprendizagem
/
Memória
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Zhongguo Zhong Xi Yi Jie He Za Zhi
Assunto da revista:
TERAPIAS COMPLEMENTARES
Ano de publicação:
2011
Tipo de documento:
Article