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Cloning and identification of microRNAs in earthworm (Eisenia fetida).
Huang, Xue-Mei; Tian, Qing-Nan; Bao, Zhen-Xia; Qin, Yun-Fei; Chen, San-Jun; Lu, Ping; Zhang, Xiao-Ling; Zhang, Yi-Zhe; Zhang, Shou-Tao.
Afiliação
  • Huang XM; Department of Bioengineering, Zhengzhou University, No. 100 of Science Road, Zhengzhou, Henan, 450001, China.
Biochem Genet ; 50(1-2): 1-11, 2012 Feb.
Article em En | MEDLINE | ID: mdl-21874588
MicroRNAs (miRNAs) (noncoding RNAs of 20-25 nucleotides) play important roles in the post-transcriptional regulation of gene expression in various eukaryotes and prokaryotes. Piwi-interacting RNAs function by combining with PIWI proteins to regulate protein synthesis and to stabilize mRNA, the chromatin framework, and genome structure. This study investigates the role of miRNAs in regeneration. A scrDNA library was constructed, and 17 noncoding RNAs from Eisenia fetida (an optimal model for the study of earthworm regeneration) were cloned and characterized. In addition, reverse transcription polymerase chain reaction was performed to analyze the expression of four small RNAs during different developmental stages. The expression levels of these RNAs in regenerating tissue were higher than in normal tissue, and the expression patterns of these small RNAs were unique during development.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oligoquetos / Regulação da Expressão Gênica no Desenvolvimento / MicroRNAs Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Biochem Genet Ano de publicação: 2012 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oligoquetos / Regulação da Expressão Gênica no Desenvolvimento / MicroRNAs Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Biochem Genet Ano de publicação: 2012 Tipo de documento: Article País de afiliação: China