Control of embryonic stem cell lineage commitment by core promoter factor, TAF3.
Cell
; 146(5): 720-31, 2011 Sep 02.
Article
em En
| MEDLINE
| ID: mdl-21884934
ABSTRACT
Deciphering the molecular basis of pluripotency is fundamental to our understanding of development and embryonic stem cell function. Here, we report that TAF3, a TBP-associated core promoter factor, is highly enriched in ES cells. In this context, TAF3 is required for endoderm lineage differentiation and prevents premature specification of neuroectoderm and mesoderm. In addition to its role in the core promoter recognition complex TFIID, genome-wide binding studies reveal that TAF3 localizes to a subset of chromosomal regions bound by CTCF/cohesin that are selectively associated with genes upregulated by TAF3. Notably, CTCF directly recruits TAF3 to promoter distal sites and TAF3-dependent DNA looping is observed between the promoter distal sites and core promoters occupied by TAF3/CTCF/cohesin. Together, our findings support a new role of TAF3 in mediating long-range chromatin regulatory interactions that safeguard the finely-balanced transcriptional programs underlying pluripotency.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Homeodomínio
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Regulação da Expressão Gênica no Desenvolvimento
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Fator de Transcrição TFIID
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Células-Tronco Embrionárias
Limite:
Animals
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Humans
Idioma:
En
Revista:
Cell
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos