Development of multiplexed species specific polymerase chain reaction assays for identification of the Culex (Melanoconion) species (Diptera: Culicidae) of the southeastern United States based on rDNA.
J Med Entomol
; 48(5): 961-6, 2011 Sep.
Article
em En
| MEDLINE
| ID: mdl-21936313
ABSTRACT
Adult female mosquitoes within the subgenus Culex (Melanoconion) Theobald (Diptera Culicidae) are difficult to identify to species using external morphological features. We present two multiplexed polymerase chain reaction assays that quickly and accurately identify specimens from the southeastern United States based on sequence differences in the internal transcribed spacers of the ribosomal DNA gene array. One assay identifies all species that occur only in Florida, whereas the second assay identifies species that may occur in other southeastern states. These assays require small amounts of DNA, such as DNA from two sonicated legs, or an individual specimen. These assays also may be run as multiple singleplex reactions to determine the mosquito species composition of virus-positive mosquito pools. Reaction volumes may be as low as 10 microl, which reduces assay cost.
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Base de dados:
MEDLINE
Assunto principal:
DNA Ribossômico
/
Reação em Cadeia da Polimerase
/
Genes de Insetos
/
Culex
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Animals
País/Região como assunto:
America do norte
Idioma:
En
Revista:
J Med Entomol
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos