Escherichia coli RNase E has a role in the decay of bacteriophage T4 mRNA.
Genes Dev
; 4(5): 873-81, 1990 May.
Article
em En
| MEDLINE
| ID: mdl-2199322
ABSTRACT
Bacteriophage T4 mRNAs are markedly stabilized, both chemically and functionally, in an Escherichia coli strain deficient in the RNA-processing endonuclease RNase E. The functional stability of total T4 messages increased 6-fold; we were unable to detect a T4 message whose functional stability was not increased. There was a 4-fold increase in the chemical stability of total T4 RNA. The degree of chemical stabilization of six specific T4 mRNAs examined varied from a maximum of 28-fold to a minimum of 1.5-fold. In the RNase E-deficient strain, several minutes delay and a slower rate of progeny production led to a reduction in final phage yield of approximately 50%. Although the effect of the rne temperature-sensitive mutation could be indirect, the simplest interpretation of our results is that RNase E acts directly in the degradation of many T4 mRNAs.
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Base de dados:
MEDLINE
Assunto principal:
Fagos T
/
RNA Mensageiro
/
RNA Viral
/
Endorribonucleases
/
Escherichia coli
Idioma:
En
Revista:
Genes Dev
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
1990
Tipo de documento:
Article
País de afiliação:
Suíça