3D magnetic resonance microscopy of the ex vivo retina.
Magn Reson Med
; 67(4): 1154-8, 2012 Apr.
Article
em En
| MEDLINE
| ID: mdl-22009721
ABSTRACT
3D-MR microscopy at 11.7T and 20 × 20 × 57 µm resolution was performed on formalin-fixed rat eyes with (I) no contrast agent and (II) Gadodiamide (Omniscan(®) ) added to the fixative. Group I data showed generally poor contrast among layers. Group II data showed markedly better lamina-specific contrast with the nerve fiber + ganglion cell layer and inner nuclear layer being hypointense, and the inner plexiform, outer plexiform, outer nuclear layer, and the segments being hyperintense. The signal-to-noise ratio in group II was higher than group I, consistent with Gadodiamide acting as a T(1) -contrast agent. All major retinal layers were assigned and their thicknesses quantified with corroboration by histology. MR microscopy allows nondestructive examination of valuable specimens and could have applications in disease and in vivo.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Retina
/
Imageamento por Ressonância Magnética
/
Imageamento Tridimensional
Limite:
Animals
Idioma:
En
Revista:
Magn Reson Med
Assunto da revista:
DIAGNOSTICO POR IMAGEM
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos