Binding of a Bolton-Hunter substituted homostatine analog to affinity-immobilized human renin.
Biochem Pharmacol
; 40(4): 765-70, 1990 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-2201301
ABSTRACT
The binding of a Bolton-Hunter reagent substituted homostatine analog, SDZ 213-776, to human renin was investigated at pH 6.5 and 7.4. At both pH values, SDZ 213-776 bound to human renin in a reversible and saturable manner. The binding characteristics conformed to a one-site binding model. The dissociation constant Kd, obtained at equilibrium, was four-fold lower at pH 6.5 that at pH 7.4 (0.94 nM vs 3.7 nM). Under non-equilibrium conditions, only the association kinetic constant k+1 was affected by pH. The results of the binding assay at pH 6.5 correlated well with those obtained in enzymatic assay at the same pH.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Oligopeptídeos
/
Renina
/
Enzimas Imobilizadas
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Biochem Pharmacol
Ano de publicação:
1990
Tipo de documento:
Article
País de afiliação:
Suíça