An LC-MS/MS method for the determination of ofloxacin in 20 µl human plasma.
J Pharm Biomed Anal
; 58: 177-81, 2012 Jan 25.
Article
em En
| MEDLINE
| ID: mdl-22018893
ABSTRACT
A sensitive and selective liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of ofloxacin in 20 µl human plasma over the concentration range of 0.078-20 µg/ml. Sample preparation was achieved by protein precipitation with acetonitrile and methanol containing the internal standard (Gatifloxacin). Chromatographic separation was achieved on a Luna 5 µm PFP (110 A, 50 × 2 mm) column with acetonitrile and water containing 0.1% formic acid (5050, v/v) as the mobile phase, at a flow rate of 400 µl/ml. The within-day and between-day precision determinations for ofloxacin, expressed as the percentage coefficient of variation, were lower than 7% at all test concentrations. Recovery of ofloxacin was greater than 70% and reproducible at the low, medium and high end of the dynamic range. No significant matrix effects were observed for the analyte or internal standard. The assay was successfully used to examine the pharmacokinetics of ofloxacin as part of a study to characterize the pharmacokinetics of a number of anti-tuberculosis drugs utilized in the treatment of multi-drug resistant tuberculosis (MDR-TB).
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ofloxacino
/
Cromatografia Líquida
/
Espectrometria de Massas em Tandem
/
Antibacterianos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Pharm Biomed Anal
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
África do Sul