Coordinated regulation of caveolin-1 and Rab11a in apical recycling compartments of polarized epithelial cells.
Exp Cell Res
; 318(2): 103-13, 2012 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-22036648
ABSTRACT
Recent studies have identified caveolin-1, a protein best known for its functions in caveolae, in apical endocytic recycling compartments in polarized epithelial cells. However, very little is known about the regulation of caveolin-1 in the endocytic recycling pathway. To address this question, in the current study we compared the relationship between compartments enriched in sub-apical caveolin-1 and Rab11a, a well-defined marker of apical recycling endosomes, using polarized MDCK cells as a model. We show that caveolin-1-containing vesicles define a compartment that partially overlaps with Rab11a, and that the distribution of subapical caveolin-1 and Rab11a shows a similar dependence on microtubule disruption. Mutants of the Rab11a effector, Rab11-FIP2 also altered the localization of caveolin-1. These findings indicate that caveolin-1 is coordinately regulated with Rab11a within the apical recycling system of polarized epithelial cells, suggesting that the two proteins are components of the same pathway.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polaridade Celular
/
Proteínas rab de Ligação ao GTP
/
Caveolina 1
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Exp Cell Res
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos