A method for analysis of lipid vesicle domain structure from confocal image data.
Eur Biophys J
; 41(2): 161-75, 2012 Feb.
Article
em En
| MEDLINE
| ID: mdl-22068825
ABSTRACT
Quantitative characterization of the lateral structure of curved membranes based on fluorescence microscopy requires knowledge of the fluorophore distribution on the surface. We present an image analysis approach for extraction of the fluorophore distribution on a spherical lipid vesicle from confocal imaging stacks. The technique involves projection of volumetric image data onto a triangulated surface mesh representation of the membrane, correction of photoselection effects and global motion of the vesicle during image acquisition and segmentation of the surface into domains using histograms. The analysis allows for investigation of the morphology and size distribution of domains on the surface.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Microscopia Confocal
/
Lipossomas Unilamelares
Idioma:
En
Revista:
Eur Biophys J
Assunto da revista:
BIOFISICA
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Dinamarca