A novel method for spatially complex diffraction-limited photoactivation and photobleaching in living cells.
J Physiol
; 590(5): 1093-100, 2012 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-22183727
ABSTRACT
Photoactivated probes have gained interest as experimental tools to study intracellular signalling pathways all the way to the molecular level. However technical limitations of the means to activate such compounds have put constraints on their use in spatially highly restricted subcellular areas. The Mosaic digital illumination system uses a high-speed array of individually addressable, tiltable micromirrors to direct continuous-wave laser light onto a specimen with diffraction-limited precision. The system, integrated into a Nikon A1R confocal microscope, was used to uncage Ca²âº or IP3 and conduct photobleaching experiments from multiple geometrically complex subcellular regions while simultaneously measuring [Ca²âº]i with high-speed confocal imaging.
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Base de dados:
MEDLINE
Assunto principal:
Cálcio
/
Miócitos Cardíacos
Limite:
Animals
Idioma:
En
Revista:
J Physiol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos