The type and extent of injuries in vitrified mouse oocytes.
Cryobiology
; 64(2): 97-102, 2012 Apr.
Article
em En
| MEDLINE
| ID: mdl-22202671
ABSTRACT
To improve the vitrification of mouse oocytes using straws, we attempted to estimate the type and extent of injuries during vitrification with a vitrification solution EAFS10/10. Injuries in oocytes were assessed based on cellular viability, the integrity of the plasma membrane, the status of the meiotic spindle/chromosomes, and morphological appearance. For morphologically normal oocytes, the ability to be fertilized and to develop into blastocysts was examined. Morphological assessment revealed 15% of oocytes to be injured by intracellular ice formed during vitrification, and 10% by osmotic swelling during removal of the cryoprotectant. When assessed by the status of spindles/chromosomes, the most sensitive criterion, damage was found in 16% of oocytes without any treatment. This value was similar to the proportion of fresh oocytes that did not cleave after insemination (13%). On exposure to EAFS10/10, the spindles/chromosomes were affected in 33% of oocytes. The exposure reduced the rate of cleavage by 18% points and the rate of development into blastocysts by 19 points. Vitrification reduced these rates by 15% and 36% points, respectively. Although the mechanism responsible for this moderate toxic effect on developmental ability is not known, information obtained in the present study will be useful to develop a practical method for the vitrification of mouse oocytes using straws.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Criopreservação
/
Vitrificação
Limite:
Animals
Idioma:
En
Revista:
Cryobiology
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China