Killing by neutrophil extracellular traps: fact or folklore?
Blood
; 119(5): 1214-6, 2012 Feb 02.
Article
em En
| MEDLINE
| ID: mdl-22210873
ABSTRACT
Neutrophil extracellular traps (NETs) are DNA structures released by dying neutrophils and claimed to constitute a new microbicidal mechanism. Killing by NET-forming cells is ascribed to these structures because it is prevented by preincubation with DNase, which has been shown to dismantle NETs, before addition of the target microorganisms. Curiously, the possibility that the microorganisms ensnared in NETs are alive has not been considered. Using Staphylococcus aureus and Candida albicans blastospores, we demonstrate that the microorganisms captured by NETs and thought to be killed are alive because they are released and recovered in cell medium by incubation with DNase. It is concluded that NETs entrap but do not kill microbes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ativação de Neutrófilo
/
Neutrófilos
Limite:
Humans
Idioma:
En
Revista:
Blood
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Itália