Delivery of membrane impermeable cargo into CHO cells by peptide nanoparticles targeted by a protein corona.
Biomaterials
; 33(9): 2746-53, 2012 Mar.
Article
em En
| MEDLINE
| ID: mdl-22226586
ABSTRACT
Nanocarriers can fulfill essential functions in the stabilization and delivery of drugs they prevent solubility issues and degradation, reduce side effects and modify the pharmacokinetic profile. However, particle based pharmaceuticals are complex and thus challenging to scale up. As formulation routines account for a large fraction of production costs, reducing complexity in the process of assembly, loading and functionalization of nanoparticles is desirable. Unlike existing approaches with similar goals, our protocol is designed to minimize usage of material and time. Prerequisite to this elegant one-step-procedure is the controlled phase-separation of a hydrophobic peptide to nanoparticles, inducing concurrent cargo-entrapment and association of a protein corona. We demonstrate the process by assembling Flutax-2 containing peptide nanoparticles functionalized with transferrin. Cellular uptake of the particles and cargo release depend on specific particle-cell interactions via transferrin receptor. These data indicate corona-mediated delivery of membrane impermeable cargo in vitro by a particulate delivery system entirely composed of amino acids.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Transferrina
/
Permeabilidade da Membrana Celular
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Sistemas de Liberação de Medicamentos
/
Nanopartículas
Limite:
Animals
Idioma:
En
Revista:
Biomaterials
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos