Peptide discovery using bacterial display and flow cytometry.
Methods Enzymol
; 503: 75-97, 2012.
Article
em En
| MEDLINE
| ID: mdl-22230566
ABSTRACT
Peptides are increasingly used as therapeutic and diagnostic agents. The combination of bacterial cell-surface display peptide libraries with magnetic- and fluorescence-activated cell sorting technologies provides an efficient and highly effective methodology to identify and engineer peptides for a growing number of molecular recognition applications. Here, detailed protocols for both the generation and screening of bacterial display peptide libraries are presented. The methods described enable the discovery and evolutionary optimization of protein-binding peptides, cell-specific peptides, and enzyme substrates for diverse biotechnology applications.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Biblioteca de Peptídeos
/
Escherichia coli
/
Descoberta de Drogas
/
Citometria de Fluxo
Tipo de estudo:
Guideline
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos