Immunogenicity characterization of two ancient wheat α-gliadin peptides related to coeliac disease.
Nutrients
; 1(2): 276-90, 2009 02.
Article
em En
| MEDLINE
| ID: mdl-22253984
ABSTRACT
The immunogenic potential of α-gliadin protein from two ancient wheats was studied with reference to coeliac disease. To this aim we investigated Graziella Ra® and Kamut® (the latter is considered an ancient relative of modern durum wheat) in comparison to four durum wheat accessions (Senatore Cappelli, Flaminio, Grazia and Svevo). ELISA and Western Blot analyses - carried out by two monoclonal antibodies raised against the α-gliadin peptides p31-49 (LGQQQPFPQQPYPQPQPF) and p56-75 (LQLQPFPQPQLPYPQPQLPY) containing a core region (underlined) reported to be toxic for coeliac patients - always showed an antibody-antigen positive reaction. For all accessions, an α-gliadin gene has also been cloned and sequenced. Deduced amino acid sequences constantly showed the toxic motifs. In conclusion, we strongly recommend that coeliac patients should avoid consuming Graziella Ra® or Kamut®. In fact their α-gliadin not only is as toxic as one of the other wheat accessions, but also occurs in greater amount, which is in line with the higher level of proteins in ancient wheats when compared to modern varieties.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Triticum
/
Doença Celíaca
/
Gliadina
Limite:
Humans
Idioma:
En
Revista:
Nutrients
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Itália