Analysis of protein posttranslational modifications using DIGE-based proteomics.
Methods Mol Biol
; 854: 129-43, 2012.
Article
em En
| MEDLINE
| ID: mdl-22311757
ABSTRACT
Difference gel electrophoresis (DIGE) is most often used to assess relative changes in the expression levels of individual proteins in multiple complex samples, and this information is valuable in making inferences about relative protein activity. However, a protein's activity is not solely dependent upon its expression level. A change in activity may also be influenced by myriad posttranslational modifications (PTMs), including palmitoylation, ubiquitination, oxidation, and phosphorylation. In this chapter, we describe the use of DIGE to determine specific PTMs by introducing specific labels or changes in pI and/or molecular weight.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Processamento de Proteína Pós-Traducional
/
Proteômica
/
Eletroforese em Gel Diferencial Bidimensional
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos