Evaluating the efficacy of subcellular fractionation of blast cells using live cell labeling and 2D DIGE.
Methods Mol Biol
; 854: 319-32, 2012.
Article
em En
| MEDLINE
| ID: mdl-22311770
ABSTRACT
Labeling of exposed cell surface proteins of live cells using CyDye DIGE fluor minimal dyes is an efficient strategy for cell surface proteome profiling and quantifying differentially expressed proteins in diseases. Here we describe a strategy to evaluate a two-step detergent-based protein fractionation method using live cell labeling followed by visualization of the fluorescently labeled cell surface proteins and fractionated proteins within a single 2D gel.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Coloração e Rotulagem
/
Células da Medula Óssea
/
Espaço Intracelular
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Eletroforese em Gel Diferencial Bidimensional
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Austrália