Using transcriptome sequencing to identify mechanisms of drug action and resistance.
Nat Chem Biol
; 8(3): 235-7, 2012 Feb 12.
Article
em En
| MEDLINE
| ID: mdl-22327403
ABSTRACT
Determining mechanisms of drug action in human cells remains a major challenge. Here we describe an approach in which multiple-drug-resistant clones are isolated and transcriptome sequencing is used to find mutations in each clone. Further analysis of mutations common to more than one clone can identify a drug's physiological target and indirect resistance mechanisms, as indicated by our proof-of-concept studies of the cytotoxic anticancer drugs BI 2536 and bortezomib.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Proto-Oncogênicas
/
Análise de Sequência de DNA
/
Proteínas Serina-Treonina Quinases
/
Proteínas de Ciclo Celular
/
Resistencia a Medicamentos Antineoplásicos
/
Transcriptoma
/
Antineoplásicos
Limite:
Humans
Idioma:
En
Revista:
Nat Chem Biol
Assunto da revista:
BIOLOGIA
/
QUIMICA
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos