Microsatellite markers for the native Texas perennial grass, Panicum hallii (Poaceae).
Am J Bot
; 99(3): e114-6, 2012 Mar.
Article
em En
| MEDLINE
| ID: mdl-22362543
ABSTRACT
PREMISE OF THE STUDY We developed microsatellites for Panicum hallii for studies of gene flow, population structure, breeding experiments, and genetic mapping. METHODS AND RESULTS:
Next-generation (454) genomic sequence data were used to design markers. Eighteen robust markers were discovered, 15 of which were polymorphic across six accessions of P. hallii var. hallii. Fourteen of the markers cross-amplified in a P. capillare accession. For the 15 polymorphic markers, the total number of alleles per locus ranged from two to 26 (mean 11.0) across six populations (11-19 individuals per population). Observed heterozygosity (mean 0.031) was 13.7 times lower than the expected heterozygosity (mean 0.426).CONCLUSIONS:
The deficit of heterozygous individuals is consistent with P. hallii having a high rate of self-fertilization. These markers will be useful for studies in P. hallii and related species.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Repetições de Microssatélites
/
Panicum
País/Região como assunto:
America do norte
Idioma:
En
Revista:
Am J Bot
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos