Efficacy of homologous inositol hexaphosphate-loaded red blood cells in sickle transgenic mice.
Br J Haematol
; 157(3): 357-69, 2012 May.
Article
em En
| MEDLINE
| ID: mdl-22404654
ABSTRACT
Patients with sickle cell disease (SCD) can present several severe symptoms during their lifetime, including painful events due to vascular occlusion (VOC). Even though multiple factors are involved in VOC, hypoxia is the most important triggering factor. Inositol hexaphosphate (IHP) reduces the oxygen-haemoglobin affinity thus improving the oxygen release in the blood stream and in the tissues. Thus, IHP-loaded homologous red blood cells (IHP-RBCs) could be able to reduce disorders in SCD. The effectiveness of treatment was assessed in two types of SCD transgenic mice (BERK and SAD). The administration of four repeated injections of IHP-RBCs in BERK mice resulted in an improved survival rate and brain development, prevention of severe anaemia and a greatly lowered risk of VOC. After one injection of IHP-RBCs, SAD mice were subjected to acute hypoxic stress. Analysis of the lungs revealed significantly decreased mRNA levels of molecules involved in intravascular disorders. Our results showed that transfusion of homologous IHP-RBCs, by increasing the oxygen delivery, reduces SCD disorders in sickle transgenic mice.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ácido Fítico
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Transfusão de Eritrócitos
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Anemia Falciforme
Tipo de estudo:
Etiology_studies
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Evaluation_studies
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Prognostic_studies
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Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Br J Haematol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
França