DyeCycle Violet used for side population detection is a substrate of P-glycoprotein.
Cytometry A
; 81(6): 517-22, 2012 Jun.
Article
em En
| MEDLINE
| ID: mdl-22407964
ABSTRACT
Cancer stem cells (CSC) are increasingly recognized as key target cells for cancer therapy because they are both tumorigenic and chemoresistant and, therefore, can give rise to recurrent disease. Side population (SP) sorting using an ultraviolet (UV) laser is an established method to isolate CSC based on ABC drug transporter-dependent (e.g., ABCG2) Hoechst 33342 efflux. We here show that Vybrant® DyeCycle™ Violet (DCV), a DNA-binding fluorophor allowing SP sorting using a non-UV laser (such as violet laser), is transported via P-glycoprotein (PgP). Because PgP might be particularly abundant in multidrug-resistant cancer cells rather than bona fide CSC, investigators using DCV should be aware that this strategy might also detect PgP-expressing non-CSC.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Neoplásicas
/
Separação Celular
/
Membro 1 da Subfamília B de Cassetes de Ligação de ATP
/
Proteínas de Neoplasias
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Cytometry A
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Áustria